Infection:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Mass Spectrometry:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Recombinant:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Protease Inhibitor:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Electron Microscopy:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Western Blot:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Bicinchoninic Acid Protein Assay:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
Reverse Transcription:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
SYBR Green Assay:Article Title: A phosphorylation-controlled switch confers cell cycle-dependent protein relocalization
Article Snippet: Lipofectamine 2000, Invitrogen (11668019); Polybrene Infection/Transfection Reagent, Sigma-Aldrich (TR-1003); GFP-Trap magnetic agarose, ChromoTek (gtma-20); Trypsin Gold Mass Spectrometry Grade, Promega (V5280); GFP recombinant protein (Invitrogen, A42613); mCherry recombinant protein (Origene, TP790040); D-biotin, Chem-Impex (00033, Cas #: 58-85-5); (+)-S-Trityl-L-cysteine, Alfa Aesar (L14384, CAS #: 2799-07-7); Puromycin dihydrochloride, Sigma-Aldrich (P8833, CAS # 58-58-2); Doxycycline hyclate, Acros (446061000, CAS #: 24390-14-5); cOmplete Protease Inhibitor Cocktail, Roche (5056489001); Sodium fluoride, Chem-Impex (01523, CAS #: 7681-49-4); Sodium orthovanadate, MP Bio (0215966410, CAS #: 13721-39-6); Oxotremorine M, Santa Cruz Biotechnology (sc-203656, CAS #: 63939-65-1); Phorbol-12-Myristate-13-Acetate, Santa Cruz Biotechnology (sc-3576, CAS #: 16561-29-8); Bryostatin 1, Cayman Chemical (14802, CAS #: 83314-01-6); Gö 6983, Cayman Chemical (13311, CAS #: 133053-19-7); Sotrastaurin, Cayman Chemical (16726, CAS #: 425637-18-9); PF 670462, Fisher Scientific (50-115-0729, CAS #: 950912-80-8); D 4476, Fisher Scientific (2902/10, CAS #: 301836-43-1); Tautomycetin, Tocris (2305, CAS #: 119757-73-2); Fostriecin, VWR (102987-176, CAS #: 87860-39-7); Okadaic acid, Cayman Chemical (10011490, CAS #: 78111-17-); Calyculin A, Santa Cruz Biotechnology (sc-24000, CAS #: 101932-71-2); Phos-tagTM Acrylamide, NARD (AAL-107); 5-gluoro-2-indolyl des-chlorohalopemide (FIPI), Cayman Chemical (13563, CAS #: 939055-18-2) 5-Hexyn-1-ol, Sigma-Aldrich (302015, CAS #: 928-90-5); AZDye 647 Azide, Click Chemistry Tools (1299); 32% Paraformaldehyde, Electron Microscopy Sciences (15714); Streptavidin Alexa Fluor 568, Invitrogen (S11226); PierceTM High Capacity Streptavidin Agarose, Thermo Fisher (20359); Streptavidin-conjugated HRP, GeneTex (GTX85912); ProLongTM Diamond Antifade Mountant with DAPI, Thermo Fisher (P36971); ClarityTM Western ECL Substrate, Bio-Rad (1705061); BCA Protein Assay Kit, Thermo Fisher (23225); Trizol (Thermo Fisher, 15596026); SMART MMLV reverse transcriptase (Takara, 639524); PerfeCTa SYBR Green SuperMix (Quantabio, 95053).
|